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KMID : 0606920100180030294
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Biomolecules & Therapeutics
2010 Volume.18 No. 3 p.294 ~ p.299
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Effect of Platycodin D on Airway MUC5AC Mucin Production and Gene Expression Induced by Growth Factor and Proinflammatory Factor
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Lee Hyun-Jae
Lee Choong-Jae
Lee Su-Yel
Jeon Byeong-Kyou
Lee Jae-Woo
Kim Young-Sik
Lee Mi-Nam
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Abstract
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In this study, we tried to investigate whether platycodin D significantly affects MUC5AC mucin production and gene expression induced by epidermal growth factor (EGF), phorbol ester (PMA) and tumor necrosis factor-α (TNF-α) from human airway epithelial cells. Confluent NCI-H292 cells were pretreated with varying concentrations of platycodin D for 30 min and then stimulated with EGF, PMA and TNF-α for 24h, respectively. MUC5AC mucin gene expression and mucin protein production were measured by RT-PCR and ELISA. The results were as follows: (1) Platycodin D was found to inhibit the production of MUC5AC mucin protein induced by EGF, PMA, and TNF-α, respectively. (2) It also inhibited the expression of MUC5AC mucin gene induced by the same inducers. These results suggest that platycodin D can regulate mucin gene expression and production of mucin protein, by directly acting on human airway epithelial cells.
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KEYWORD
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Airway mucin, MUC5AC, Platycodin D
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